Each chapter will have a bipartite structure: the first part establishes the fundamental physics concepts and describes the instrumentation or technique, while the second illustrates current applications in addressing complex questions in biology.In concept, once a material system absorbs the incident light of some energy or wavelength, part of that energy is transferred to the system for various internal conversion needs, and a very short time later the other part is converted to light emission at a slightly lower energy (longer wavelength).The internal transfer part is very rapid and radiationless, but dephasing.
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